Malondialdehyde Level Profile and Liver Histology as a Marker of Cellular damage in Male Mice (Mus musculus) Supplemented With Zink
DOI:
https://doi.org/10.35747/hmj.v6i2.514Keywords:
Cyclophosphamide, Histology, Liver, ZincAbstract
Zinc as an antioxidant could bind metallothionein to protect the body from the effects of anti-cancer drugs. Cyclophosphamide) which is used to treat cancer can cause side effects. Cellular CP works through the depletion mechanism of the GSH pathway which produces free radicals with activation by copper or iron in the body that damages liver tissue. This study was conducted to determine the effect of zinc on malondialdehyde levels and histopathological features of the liver of mice induced by cyclophosphamide. This study used 24 male mice divided into 6 groups. The negative control group was not given zinc and cyclophosphamide, the positive control I was only given zinc, the positive control II was only given cyclophosphamide, and the 3 treatment groups were given cyclophosphamide 200 mg/Kg and zinc at doses of zinc 25 mg/Kg, 50 mg/Kg respectively and 100 mg/Kg. The treatment was carried out for 35 days followed by surgery, liver sampling, MDA examination, and preparation HE staining. Liver tissue damage was assessed using MDA levels and the Manja-Roenigk score. The results of the One Way ANOVA test show a significance value (p = 0.000). The most effective dose of zinc to repair damage to the liver tissue of mice is 100 mg/Kg. The conclusion of this study shows that zinc affects improving the liver tissue of mice induced by cyclophosphamide
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