Comparison of To tal Flavonoid Levels Based on Differences in Ethanol Solvent Concentration from Kapok Randu Leaf Extract (Ceiba pentandra L.)
Abstract
Kapok leaves are empirically used as traditional medicine to treat diarrhea. The secondary metabolite compounds contained in kapok leaves which function as antibacterial inhibitors are flavonoids. In this study using a true experimental research design. The difference in concentration also affects the extraction yield. Knowing the total flavonoid content of kapok leaves (Ceiba Pentandra L.) based on differences in solvent concentrations of 60%, 70% and 96% ethanol as well as analyzing the relationship and effect of solvent concentration on total flavonoid levels of kapok leaves. In this study the instrument used was a uv-vis spectrophotometer, this instrument was used to measure total flavonoid levels in kapok leaves. In qualitative research it was used to identify the content of secondary metabolite compounds of flavonoids by looking at changes in color from the results of the reagent test for the object studied with the Thin Layer Chromatography (TLC) instrument. The maceration extraction method was then tested for phytochemical screening using color test and thin layer chromatography (TLC) on kapok leaf extract to identify flavonoid compounds. To determine the total flavonoid content, the levels were tested using UV-Vis Spectrophotometry. Then use data analysis with Kruskal Wallis to see the effect.The results of phytochemical screening with color tests and Thin Layer Chromatography of kapok leaves were positive for flavonoid compounds. The results showed that there was an effect of differences in solvent concentration on total flavonoid levels.Research with the color reaction test of all the solvents used was positive for flavonoid compounds, as well as the concentration test carried out with 70% ethanol solvent concentration was more effective
References
Agustina R, Agustin L, Priyadi S. 2020. Validasi Metode Analisis Total Flavonoid Content Menggunakan Spectrofotometer UV/VIS Jurusan Teknik Kimia Di Politeknik Negeri Malang. Jurnal Teknik: Ilmu dan Aplikasi, Vol. 8(1), Hal: 34-41.
Ahmad AR, Juwita, Afrianty S, Ratulangi D, Malik A. 2015. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharm Sci Res. Universitas Muslim Indonesia. ISSN: 2407-2354. Vol. 2 No. 1
Candra, L. M. M., Andayani, Y., & Wirasisya, D. G. (2021). Pengaruh Metode Ekstraksi Terhadap Kandungan Fenolik Total dan Flavonoid Total Pada Ekstrak Etanol Buncis (Phaseolus vulgaris L.). Jurnal Pijar Mipa, 16(3), 397. https://doi.org/10.29303/jpm.v16i3.2308
Fajriaty Inarah, IH Hariyanto, Andres, Setyaningrum R. 2018. Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Dari Ekstrak Etanol Daun Bintagur (Calophyllum soulattri Burm. F.). Jurnal Pendidikan
Informatika dan Sains, Vol. 7(1), Hal: 54-67.
Febrianti D. R, Khairina N, Alisa P. N. 2018. Uji Aktivitas Anti Mikroorganisme Ekstrak Jeringau (Acorus calanus L.) Terhadap Jamur Candida albicans Dan Bakteri Staphylococcus aureus. Jurnal Insan Farmasi Indonesia, Vol. 1(1), Hal: 96-103.
Harborne JB. 1987. Metode Fitokimia Tumbuhan. Penerbit ITB. Bandung. Tersedia pada : http://lib.ui.ac.id/detail.jsp?id=20284797.
Hasmawati, Hardianti, M., & H, J. (2020). Teh Herbal Innovation (Teunkap) Upaya Pemanfaatan Daun Kapuk sebagai Pencegahan Penyakit Maag di Kabupaten Maros. Jurnal Pena, 7(1), 64–71. https://journal.unismuh.ac.id/index.php/pena/article/view/3332
Hikmawanti N. P. E, Hariyanti, Aulia C., Viransa V. P. 2016. Kandungan Piperin Dalam Ekstrak Buah Lada Hitam Dan Buah Lada putih (Piper nigrum L.) Yang Diekstraksi Dengan Variasi Konsentrasi Etanol Menggunakan Metode KLT-Densitometri. Media Farmasi Vol. 13(2). Hal: 173-185.
Irawan, A. (2019). Kalibrasi Spektrofotometer Sebagai Penjaminan Mutu Hasil Pengukuran dalam Kegiatan Penelitian dan Pengujian. Indonesian Journal of Laboratory, 1(2), 1. https://doi.org/10.22146/ijl.v1i2.
Malik, A. A., Prayudha S, J., Anggreany, R., Sari, M. W., & Walid, A. (2021). Keanekaragaman Hayati Flora Dan Fauna Di Kawasan Taman Nasional Bukit Barisan Selatan (Tnbbs) Resort Merpas Bintuhan Kabupaten Kaur. DIKSAINS : Jurnal Ilmiah Pendidikan Sains, 1(1), 35–42. https://doi.org/10.33369/diksains.v1i1.14702 Maskura N. 2021. Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol Dari Ekstrak Daun Suruhan (Peperomia Pellucida L. Kunth). [Skripsi]. Universitas Sari Mulia.
Banjarmasin.
Notoatmodjo, S. (2014). Metodologi Penelitian Kesehatan. PT Rineka Cipta.
Purwanti, A., Aziz, A., R, A. D. dan, & Riyadi, F. (2015). Pemanfaatan Hasil Alam ( Daun Randu Dan Daun Jambu Biji ) sebagai Antidiare. J Teknologi, Industri Dan Informasi, 10(1), 753–758.
Riane Yuswi, N. C. (2017). Ekstrasi Antioksidan Bawang Dayak (Eleutherine palmifolia) dengan Metode Ultrasonic Bath (Kajian Jenis Pelarut dan Lama Ekstraksi). Jurnal Pangan Dan Agroindustri, 5(1), 71– 78.
Rina Pratiwi Hidayati. 2014. Potensi Kapuk Randu (Ceiba pentandra gaertn.) Dalam Penyediaan Obat Herbal. E-Journal WIDYA Kesehatan dan Lingkungan. Vol(1)Nomor(1), Hal 53-60.
